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  • Title: A new real-time RT-PCR targeting VP4-VP2 to detect and quantify enterovirus D68 in respiratory samples.
    Author: Royston L, Geiser J, Josset L, Schuffenecker I, Tapparel C.
    Journal: J Med Virol; 2017 Oct; 89(10):1879-1881. PubMed ID: 28169437.
    Abstract:
    Causing an international outbreak of respiratory disease, Enterovirus D68 quickly entered the closed circle of emerging viral pathogens of public health significance. As rapid and accurate detection of EV-D68 is essential for an efficient clinical management, we designed and validated a new highly efficient one-step quantitative rRT-PCR specific to EV-D68 VP4-VP2 region. With 100% specificity and 95.6% sensitivity to all EV-D68 strains, this new assay can be reliably used to detect and quantify EV-D68 in respiratory samples and represents an interesting additional tool for diagnosis as it targets an original region of the genome.
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