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  • Title: The identification of 5,6-dihydrouridine in normal human urine by combined gas chromatography/mass spectrometry.
    Author: Reimer ML, Schram KH, Nakano K, Yasaka T.
    Journal: Anal Biochem; 1989 Sep; 181(2):302-8. PubMed ID: 2817393.
    Abstract:
    The identification of 5,6-dihydrouridine in normal human urine is reported. Partial purification and isolation of the compound by boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography preceded its characterization as a trimethylsilyl derivative by combined gas chromatography/mass spectrometry. Structure proof is based upon a comparison of mass spectral and chromatographic features of the urinary component to that of an authentic reference sample. Additional data derived from high resolution mass measurements and deuterium isotope-labeling experiments provide confirmation of fragment ion structure. The poor detectability inherent in the HPLC/uv analysis of nucleosides is also discussed.
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