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  • Title: [Analysis of erythrocytic carrier molecules (glycosphingolipids) with ABO and Le specificity].
    Author: Hauser R, Rand S, Brinkmann B.
    Journal: Beitr Gerichtl Med; 1989; 47():149-52. PubMed ID: 2818472.
    Abstract:
    Membrane structures (stroma) were obtained from erythrocytes of various ABO and Le blood groups using a butanol/phosphate buffer extraction. The extracts were separated using thin layer chromatography and the ABO and Le antigens demonstrated directly on the chromatogram using the PAP-technique. Detection was carried out using either Ulex europaeus lectin or monoclonal antibodies. By this method the direct staining of the bands allowed a specific demonstration of the ABH and Leactive glycosphingolipids. A determination of the number of sugar residues is possible as well as the recognition of quantitative variants.
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