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  • Title: The effect of nucleotide substitution on DNA denaturation profiles.
    Author: Razlutskii IV, Shlyakhtenko LS, Lyubchenko YuL.
    Journal: Nucleic Acids Res; 1987 Aug 25; 15(16):6665-76. PubMed ID: 2819824.
    Abstract:
    The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28 +/- 0.03C upon the deletion of one GC pair. The melting of DNA fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.
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