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  • Title: High level expression of the EcoP1 modification methylase gene and characterisation of the gene product.
    Author: Hornby DP, Müller M, Bickle TA.
    Journal: Gene; 1987; 54(2-3):239-45. PubMed ID: 2820845.
    Abstract:
    We have cloned the gene coding for the EcoP1 modification methylase in an expression system based on the phage lambda pL promoter and the cI857-coded thermoinducible repressor. We have used this system to purify the enzyme on the 20-30-mg scale and have examined some of its enzymatic properties. The enzyme is a tetramer of Mr 72,000 subunits and is approx. 40% alpha-helical. Experiments with the methyl donor, S-adenosyl methionine, radioactively labelled in different positions indicate that a methyl group is transferred to the enzyme during the reaction in what is most likely a covalent bond.
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