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  • Title: Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization.
    Author: Ticehurst JR, Feinstone SM, Chestnut T, Tassopoulos NC, Popper H, Purcell RH.
    Journal: J Clin Microbiol; 1987 Oct; 25(10):1822-9. PubMed ID: 2822759.
    Abstract:
    Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA.
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