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  • Title: Kinetics of pump currents generated by the Na+,K+-ATPase.
    Author: Fendler K, Grell E, Bamberg E.
    Journal: FEBS Lett; 1987 Nov 16; 224(1):83-8. PubMed ID: 2824243.
    Abstract:
    Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s-1 (22 degrees C). The exponential components found in the transient currents are compared to transitions in the Albers-Post scheme.
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