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  • Title: A highly sensitive detection of chloramphenicol based on chemiluminescence immunoassays with the cheap functionalized Fe3 O4 @SiO2 magnetic nanoparticles.
    Author: Linyu W, Manwen Y, Chengzhi F, Xi Y.
    Journal: Luminescence; 2017 Sep; 32(6):1039-1044. PubMed ID: 28276194.
    Abstract:
    A strategy has been applied to chloramphenicol (CAP) detection with chemiluminescence immunoassays (CLIA) based on cheap functionalized Fe3 O4 @SiO2 magnetic nanoparticles (Fe-MNPs). The strategy that bovine serum albumin (BSA) was immobilized on cheap functionalized Fe-MNPs and that the CAP molecules were then immobilized on BSA, avoided the long process of dialysis for preparation of the BSA-CAP conjugates. The samples were detected for both methods that utilized two different kinds of functionalized Fe-MNPs (amine-functionalized Fe3 O4 @SiO2 and carboxylic acid-functionalized Fe3 O4 @SiO2 ). The sensitivities and limits of detection (LODs) of the two methods were obtained and compared based on inhibition curves. The 50% inhibition concentrations (IC50 ) values of the two methods were about 0.024 ng ml-1 and 0.046 ng ml-1 respectively and LODs were approximately 0.0002 ng ml-1 and 0.001 ng ml-1 respectively. These methods were much more sensitive than that of any traditional enzyme-linked immunosorbent assay (ELISA) previously reported. Therefore, such chemiluminescence methods could be easily adapted for small molecule detection in a variety of foods using Fe-MNPs.
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