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  • Title: Diurnal variation in 5'-nucleotidase activity in rat liver. A quantitative histochemical study.
    Author: Frederiks WM, Marx F, Bosch KS, Van Noorden CJ.
    Journal: Histochemistry; 1987; 87(5):439-43. PubMed ID: 2828284.
    Abstract:
    The diurnal variation of 5'-nucleotidase activity in periportal and pericentral areas of rat liver parenchyma has been determined with quantitative histochemical means. 5'-Nucleotidase activity was estimated using microdensitometry in cryostat sections after being incubated with a medium according to Wachstein and Meisel (1957). It appeared that 5'-nucleotidase activity was significantly higher in pericentral areas than in periportal areas throughout the daily cycle and showed a maximum at the end of the light period. It was concluded that 5'-nucleotidase activity may be related with the capacity to diminish messenger RNA resulting in protein breakdown.
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