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  • Title: Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens.
    Author: Swenson PD, Kaplan MH.
    Journal: J Clin Microbiol; 1987 Dec; 25(12):2445-6. PubMed ID: 2828421.
    Abstract:
    The conventional virus isolation technique was compared with a 24-h shell vial centrifugation culture technique and with a 48-h tube culture method for the detection of cytomegalovirus (CMV) in MRC-5 cells. Of 200 clinical specimens tested, 41 were positive for CMV by at least one procedure. Indirect immunoperoxidase staining was positive for 32 (78.0%) of 41 specimens in the tube culture method and for 30 (73.2%) of 41 specimens in the shell vial centrifugation method. CMV was detected in 23 (56.1%) of 41 specimens by the development of cytopathic effect within 14 days.
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