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  • Title: [Effective selection and analysis of IS1 element transposition using the oLpLN region of phage lambda].
    Author: Glukhov IL, Fodor I.
    Journal: Mol Gen Mikrobiol Virusol; 1987 Sep; (9):27-30. PubMed ID: 2828939.
    Abstract:
    The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element insertion. The frequency of IS1 transposition into pL depends on genotype. The plasmid pNT6 may be considered to be a useful target DNA for screening and analysis of IS1-element transposition.
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