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  • Title: Relationship between the electrochemical proton gradient and respiration: pyruvate and l-palmitoylcarnitine as hydrogen donors for rat heart mitochondria.
    Author: Schönfeld P, Petzold D.
    Journal: Biomed Biochim Acta; 1987; 46(8-9):S549-52. PubMed ID: 2829871.
    Abstract:
    The electrochemical proton gradient (delta/uH+) and the rate of respiration were determined as function of the work load at conditions where isolated rat heart mitochondria utilize pyruvate and/or l-palmitoyl-carnitine as hydrogen donors. Delta/uH+ was calculated from the distribution of 86Rb+ (delta psi marker) and [14C]-dimethyloxazolidine-2,4-dione (delta pH marker) between the mitochondrial matrix space and the medium. A transition from l-palmitoylcarnitine to pyruvate utilization did not change the rate of hydrogen supply or the mitochondrial energy state.
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