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  • Title: Dried blood spot validation of five immunosuppressants, without hematocrit correction, on two LC-MS/MS systems.
    Author: Koster RA, Veenhof H, Botma R, Hoekstra AT, Berger SP, Bakker SJ, Alffenaar JC, Touw DJ.
    Journal: Bioanalysis; 2017 Apr; 9(7):553-563. PubMed ID: 28300419.
    Abstract:
    AIM: Hematocrit (Ht) effects remain a challenge in dried blood spot (DBS) sampling. The aim was to develop an immunosuppressant DBS assay on two LC-MS/MS systems covering a clinically relevant Ht range without Ht correction. RESULTS: The method was partially validated for tacrolimus, sirolimus, everolimus, cyclosporin A and fully validated for mycophenolic acid on an Agilent and Thermo LC-MS/MS system. Bias caused by Ht effects were within 15% for all immunosuppressants between Ht levels of 0.23 and 0.48 l/l. Clinical validation of DBS versus whole blood samples for tacrolimus and cyclosporin A showed no differences between the two matrices. CONCLUSION: A multiple immunosuppressant DBS method without Ht correction, has been validated, including a clinical validation for tacrolimus and cyclosporin A, making this procedure suitable for home sampling.
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