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  • Title: [Isolation of a stable cell line after transfection of rat embryo fibroblasts by the gene of the polyomavirus large T antigen].
    Author: Komissarova EV, Revazova ES, Kiselev FL.
    Journal: Biull Eksp Biol Med; 1988 Mar; 105(3):347-9. PubMed ID: 2832020.
    Abstract:
    A stable cell line REF(LT) was established upon transfection of DNA plasmid containing a large T gene of polyoma virus. REF(LT) cells grow in a monolayer, their growth depends on the underlayer, they are non-carcinogenic. The dependence of cellular growth on serum factors is decreased.
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