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  • Title: Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis.
    Author: van den Berg FM, Jiwa M, Rook R, Geelen JL.
    Journal: J Gen Virol; 1988 Mar; 69 ( Pt 3)():699-704. PubMed ID: 2832532.
    Abstract:
    High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.
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