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  • Title: Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.
    Author: Bowring FJ, Yeadon PJ, Catcheside DEA.
    Journal: Methods Mol Biol; 2017; 1471():133-145. PubMed ID: 28349393.
    Abstract:
    We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.
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