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  • Title: Tritrichomonas foetus: freeze-fracture cytochemistry using polymyxin B.
    Author: Benchimol M, De Souza W.
    Journal: Exp Parasitol; 1988 Jun; 66(1):35-43. PubMed ID: 2835258.
    Abstract:
    The general structure of Tritrichomonas foetus incubated in the presence of the peptide antibiotic polymyxin B, which interacts specifically with anionic phospholipids, was analyzed using transmission electron microscopy of thin sections, and freeze-fracture replicas. Polymyxin B induced morphological changes in the plasma membrane of the parasites with the formation of membrane blebs with a diameter varying from 65 nm to 1.5 micron. Freeze-fracture images of the membrane lining the blebs showed that their inner membrane half is smooth. However, membrane particles, with a density similar to that observed on the E face of the plasma membrane, were seen on the outer half of this membrane.
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