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  • Title: Chemical reactions within DNA duplexes. Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent.
    Author: Sokolova NI, Ashirbekova DT, Dolinnaya NG, Shabarova ZA.
    Journal: FEBS Lett; 1988 May 09; 232(1):153-5. PubMed ID: 2835262.
    Abstract:
    Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution. Optimum conditions for this vigorous and effective reaction were developed. CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes.
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