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  • Title: [Effects of dexamethasone on MRL/lpr mice with systemic lupus erythematosus complicated with cognitive dysfunction].
    Author: Wang Y, Tang J, Shen L, Li J, Zha C, Wang R, Hu K, Xi J, Chang J, Xie C.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2017 Mar 28; 42(3):251-256. PubMed ID: 28364096.
    Abstract:
    To evaluate the effects of dexamethasone on systemic lupus erythematosus complicated with cognitive dysfunction.
 Methods: Ten wild type mice and 20 MRL/lpr mice were applied for the research. MRL/lpr mice were randomly assigned to a MRL/lpr group and a MRL/lpr + dexamethasone (1.5 mg/kg) group. Interleukin-6 (IL-6), IL-1β, and tumor necrosis factor alpha (TNF-α) in serum and hippocampus were detected. The protein phosphorylation levels of phosphoinositide 3-kinase (P-PI3K), protein kinase B (P-Akt), NF-kappa-B inhibitor alpha (P-IκBa) and nuclear transcription factor 
kappa-B p65 (P-NF-κB p65) were detected by Western blot, the level of P-NF-κB p65 also was detected by immunohistochemistry. 
 Results: Treatment with dexamethasone (1.5 mg/kg) alleviated the cognitive dysfunction and decreased the levels of IL-6, IL-1β and TNF-α in serum and hippocampus, and reduced the levels of P-PI3K, P-Akt, P-IκBa and P-NF-κB p65 in hippocampus in MRL/lpr mice.
 Conclusion: Dexamethasone may play a protective role in the cognitive function by decreasing the levels of TNF-α and IL-1β in the hippocampus of MRL/lpr lupus mice. 目的：探讨地塞米松对MRL/lpr狼疮小鼠认知功能的影响。方法：MRL/lpr狼疮小鼠随机分为MRL/lpr组、MRL/lpr+地塞米松(1.5 mg/kg)组，每组10只；野生型正常对照组C57BL/6小鼠10只。采用 Morris水迷宫观察各组小鼠认知功能和行为。检测各组小鼠血清、海马组织中炎症因子白细胞介素-6(interleukin-6，IL-6)、白细胞介素-1β(interleukin-1β，IL-1β)、肿瘤坏死因子-α(tumor necrosis factor，TNF-α)含量；检测各组小鼠海马组织中磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase，PI3K)/蛋白激酶B(protein kinase B，AKT)/核因子-κB p65(nuclear factor-kappa- binding p65，NF-κB p65)相关蛋白P-PI3K，P-Akt和磷酸化核转录因子κB抑制蛋白a(phospho-inhibitor of nuclear factor kappa Ba，P-IκBa)及P-NF-κB p65的表达。免疫组织化学法观察各组小鼠海马区P-NF-κB p65阳性表达。结果：地塞米松能缓解MRL/lpr狼疮小鼠认知功能障碍，降低血清及海马组织IL-6，IL-1β，TNF-α的表达；抑制 P-PI3K，P-Akt，P-IκBa和P-NF-κB p65的表达。结论：地塞米松可能通过降低MRL/lpr狼疮小鼠海马区TNF-α和IL-1β水平，从而对其认知功能起保护作用。.
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