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  • Title: Improved xylitol production by expressing a novel d-arabitol dehydrogenase from isolated Gluconobacter sp. JX-05 and co-biotransformation of whole cells.
    Author: Qi X, Zhang H, Magocha TA, An Y, Yun J, Yang M, Xue Y, Liang S, Sun W, Cao Z.
    Journal: Bioresour Technol; 2017 Jul; 235():50-58. PubMed ID: 28364633.
    Abstract:
    In the present study, a novel ardh gene encoding d-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26.1g/L xylitol was produced from 30g/L d-arabitol in 22h with a yield of 0.87g/g. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp. alone, and was improved 10.1% than that of Gluconobacter sp. mixed with BL21-xdh.
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