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  • Title: Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus.
    Author: Keen CL, Mendelovitz S, Cohen G, Aharonowitz Y, Roy KL.
    Journal: Mol Gen Genet; 1988 Apr; 212(1):172-6. PubMed ID: 2836705.
    Abstract:
    A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.
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