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  • Title: Evidence for specific binding of 15 hydroxyeicosatetraenoic acid to pituitary rat cells.
    Author: Rabier M, Crastes de Paulet A, Damon M.
    Journal: Biochem Biophys Res Commun; 1988 May 16; 152(3):1179-84. PubMed ID: 2837180.
    Abstract:
    Specific receptors for [3H]-15 HETE have been identified on GH3 cells, a cloned strain of rat pituitary cells. With incremental inputs of radioligand and a constant cell number, specific [3H]-15 HETE binding reached a plateau indicative of saturable binding sites. Ligand analysis of the Scatchard plot demonstrated a single class of high affinity binding sites with a dissociation constant (Kd) of 0.75 nM. 12 HETE competed with radiolabeled 15 HETE (IC50 = 1 x 10(-6) +/- 0.8 M). In contrast, arachidonic acid, leukotriene B4, prostaglandins E2 and F2 alpha did not compete with [3H]-15 HETE.
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