These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Exotoxin A of Pseudomonas aeruginosa: evidence that domain I functions in receptor binding.
    Author: Guidi-Rontani C, Collier RJ.
    Journal: Mol Microbiol; 1987 Jul; 1(1):67-72. PubMed ID: 2838729.
    Abstract:
    We have constructed defined deletions in the structural gene of Pseudomonas aeruginosa exotoxin A (ETA) in order to probe the function of Domain I of this protein. Three forms of the gene containing specific deletions were expressed in a strain of Escherichia coli K12 with lesions in the htpR and Ion genes; extracts containing the gene products were tested for ADP-ribosylation activity, cytotoxicity, and ability to protect sensitive cells from the cytotoxic action of authentic ETA. Two of the mutant ETAs gave concentration-dependent protection against authentic ETA, and protection correlated with the presence of the bulk of Domain I. The results support the notion that Domain I functions in binding the toxin to specific cell-surface receptors.
    [Abstract] [Full Text] [Related] [New Search]