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  • Title: Altered localisation of the precursor of a secreted protein in E. coli by a carboxyl-deletion.
    Author: Burns DM, Beacham IR.
    Journal: Biochem Int; 1987 Jun; 14(6):1073-7. PubMed ID: 2839184.
    Abstract:
    The effect on protein localisation of a carboxy-terminal deletion of periplasmic UDP-glucose hydrolase (5'-nucleotidase) has been determined in vivo using immunoprecipitation. The processed form of the truncated protein was found in the periplasm; however, in contrast to the situation with the normal protein, the preprotein form of the truncated protein was also found in the periplasm. This result confirms previous semi-in vitro data and supports the suggestion that a conformational change in a membrane-bound intermediate is a normal part of the secretory pathway.
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