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  • Title: Highly Sensitive Detection of Bisphenol A by NanoAptamer Assay with Truncated Aptamer.
    Author: Lee EH, Lim HJ, Lee SD, Son A.
    Journal: ACS Appl Mater Interfaces; 2017 May 03; 9(17):14889-14898. PubMed ID: 28393521.
    Abstract:
    For the sensitive quantification of bisphenol A (BPA), we have developed NanoAptamer assay, which employs aptamer and complementary signaling DNA, a set of quantum dots (QD), and magnetic beads (MBs). Signaling DNA-QD655 was tethered to MB-QD565 via the aptamer. The affinity of the aptamer to BPA resulted in the release of the signaling DNA-QD655 from the complex and hence the corresponding decrease in the QD655 fluorescence measurement signal. Three new aptamers (23, 58, and 24-mer) were designed via truncation of the reference aptamer (73-mer). The sensitivity and selectivity of each aptamer for BPA detection via NanoAptamer assay were investigated. One of the truncated aptamers (24-mer) has shown a significantly better performance (limit of detection, LOD, 0.17 pg/mL) than the reference 73-mer aptamer (LOD, 570 pg/mL). It has also shown the best selectivity for BPA detection over BPA analogues (i.e., bisphenol B, bisphenol C, and diethylstilbestrol). It corresponded to a normalized fluorescence change of 33.7% at the environmentally relevant concentration of 1 ng/mL (1 ppb) BPA; however, the analogues remained unchanged (2.3-3.9%).
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