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  • Title: Inversion events in the HSV-1 genome are directly mediated by the viral DNA replication machinery and lack sequence specificity.
    Author: Weber PC, Challberg MD, Nelson NJ, Levine M, Glorioso JC.
    Journal: Cell; 1988 Jul 29; 54(3):369-81. PubMed ID: 2840204.
    Abstract:
    The bacterial transposable element Tn5 was observed to undergo high-frequency sequence inversion when integrated into the herpes simplex virus type 1 (HSV-1) genome. Deletion analysis of the IS50 elements through which this recombination event occurred demonstrated the absence of cis-acting signals involved in the inversion process. Several observations suggested an intimate association of the recombination mechanism with HSV-1 DNA replication, including the ability of the seven viral genes that are essential for HSV-1 DNA synthesis to mediate Tn5 inversion in the absence of any other viral functions. Comparable results were obtained by using duplicate copies of the L-S junction of the HSV-1 genome. Thus inversion of the L and S components of the HSV-1 genome during productive infection does not appear to be a site-specific process, but rather is the result of generalized recombination mediated by the complex of gene products that replicate the viral DNA.
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