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  • Title: Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids.
    Author: Mendoza MC, Gonzalez AJ, Mendez FJ, Hardisson C.
    Journal: Eur J Epidemiol; 1988 Jun; 4(2):158-63. PubMed ID: 2841150.
    Abstract:
    We conducted a surveillance program on epidemic and/or endemic Shigella strains in Asturias (Spain), their frequency and dispersion in our community, and their R-plasmids. We analyzed initial isolates of Shigella sonnei from two epidemic outbreaks using antibiotic resistance patterns and plasmid profile analysis as epidemiological markers. We found that the 2 outbreaks were caused by different S. sonnei strains, which respectively carried one and two R-plasmids together with other plasmids. The molecular relationship among these and three other R-plasmids from two S. sonnei strains isolated during a previous outbreak, were studied by restriction enzyme analysis and DNA-DNA hybridizations. We were able to establish different levels of relationship among the six R-plasmids.
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