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  • Title: Overproduction, purification and crystallization of TaqI restriction endonuclease.
    Author: Barany F.
    Journal: Gene; 1988 May 30; 65(2):167-77. PubMed ID: 2842231.
    Abstract:
    Under phoA promoter control, TaqI endonuclease was overproduced to 5% of Escherichia coli cellular proteins. This was achieved by fusing the endonuclease gene to the first four codons of the alkaline phosphatase signal sequence. For maximal overproduction (30% of cellular proteins), a putative 14-bp hairpin within the endonuclease coding sequence was replaced with degenerate codons. In addition, TaqI methylase was required to protect host DNA. The endonuclease was purified in sufficient amounts for crystallization.
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