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  • Title: [Cloning of terminal fragments of the avian adenovirus CELO genome and isolation of a recombinant plasmid carrying the viral oncogene].
    Author: Krugliak VA, Akopian TA, Naroditskiĭ BS, Kartashova IM, Shevliagin VIa.
    Journal: Mol Gen Mikrobiol Virusol; 1988 May; (5):27-30. PubMed ID: 2842669.
    Abstract:
    The terminal fragment of avian adenovirus CELO has been cloned in a plasmid vector. The obtained recombinant plasmid pCBE1 carries the terminal BamHI-E fragment of CELO DNA. Transfection of a nonpermissive culture of Rat2 cell line by the plasmid DNA results in formation of transformation focuses. The cloned BamHI-E fragment of CELO DNA is concluded to contain the viral oncogene. Thus, the CELO genome region deriving the BamHI-E fragment is "left".
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