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  • Title: Human leukocyte interferon: production, purification to homogeneity, and initial characterization.
    Author: Rubinstein M, Rubinstein S, Familletti PC, Miller RS, Waldman AA, Pestka S.
    Journal: Proc Natl Acad Sci U S A; 1979 Feb; 76(2):640-4. PubMed ID: 284389.
    Abstract:
    A method of fractionating proteins by high-performance liquid partition chromatography has been developed and used for isolation and purification to homogeneity of one of the species of human leukocyte interferon. The homogeneous interferon exhibited a sharp peak on high-performance liquid chromatography and a single narrow band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Extraction of the gel gave a single sharp peak of antiviral activity coinciding with the protein band. The specific activity of pure interferon was found to be 2--4 X 10(8) units/mg, based on amino acid analysis. The molecular weight is 17,500--18,000.
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