These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Comparison of bovine platelet and teat alpha 2-adrenoceptor characteristics by [3H]rauwolscine binding.
    Author: Roets E, Burvenich C, Peeters G.
    Journal: Arch Int Pharmacodyn Ther; 1988; 293():175-85. PubMed ID: 2844128.
    Abstract:
    Alpha 2-adrenoceptors on platelet membranes, isolated from "slaughterhouse cows," were characterized by [3H]-rauwolscine binding. There were 30 +/- 3 [3H]rauwolscine sites per platelet, corresponding to 194 +/- 18 fmol/mg protein. These had an equilibrium dissociation constant (KD) of 5.25 +/- 0.56 nM, a Hill coefficient of 1.00 +/- 0.01 (n = 9), and were competed for by adrenergic compounds stereoselectively. The rank order of catecholamines coincided with that expected for alpha 2-adrenoceptors. Ki-values for inhibition of specific [3H]rauwolscine binding by 7 agonists and 6 antagonists were in the same range as previously observed in teat tissue. It is therefore concluded that quite similar [3H]rauwolscine binding sites were identified and characterized in both bovine teats and platelets. Direct comparison of binding characteristics between alpha 2-adrenoceptors from teat tissue and platelets of the same "primiparous cows," revealed no difference in binding affinity, although a significant higher platelet receptor number was noted in the platelets (platelets: 353 fmol/mg protein; teat tissue: 91 fmol/mg protein; n = 10).
    [Abstract] [Full Text] [Related] [New Search]