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  • Title: Fluorescent coumarin-based probe for cysteine and homocysteine with live cell application.
    Author: Wei LF, Thirumalaivasan N, Liao YC, Wu SP.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2017 Aug 05; 183():204-208. PubMed ID: 28454072.
    Abstract:
    Cysteine (Cys) and homocysteine (Hcy) are two of important biological thiols and function as important roles in several biological processes. The development of Cys and Hcy probes will help to explore the functions of biothiols in biological systems. In this work, a new coumarin-based probe AC, containing an acryloyl moiety, was developed for Cys and Hcy detection in cells. Cys and Hcy undergo a nucleophilic addition and subsequent cyclization reaction to remove to the acryloyl group and yield a fluorescent product, 7-hydroxylcomuarin. The probe AC showed good selectivity for cysteine and homocysteine over glutathione and other amino acids and had low detection limits of 65nM for Cys and 79nM for Hcy, respectively. Additionally, confocal imaging experiments demonstrated that the probe AC can be applied to visualize Cys and Hcy in living cells.
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