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  • Title: Derivatization and Gas Chromatography of Fatty Acids from Yeast.
    Author: Knittelfelder OL, Kohlwein SD.
    Journal: Cold Spring Harb Protoc; 2017 May 01; 2017(5):. PubMed ID: 28461653.
    Abstract:
    Analysis of fatty acids by gas chromatography (GC) is facilitated by the generation of volatile fatty acid methyl ester (FAME) derivatives. Here we describe the esterification procedure and a typical program for separating FAMEs using a gas chromatograph equipped with a flame ionization detector or a dual stage quadrupole-mass spectrometry detector. GC is a rather simple technique that provides quantitative information on cellular fatty acid content and composition by use of an internal fatty acid standard.
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