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  • Title: Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences.
    Author: Melki R, Khoury B, Catalan F.
    Journal: J Med Virol; 1988 Oct; 26(2):137-43. PubMed ID: 2846775.
    Abstract:
    We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.
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