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  • Title: 31P magnetization transfer in the phosphoglyceromutase-enolase coupled enzyme system.
    Author: Chapman BE, Stewart IM, Bulliman BT, Mendz GL, Kuchel PW.
    Journal: Eur Biophys J; 1988; 16(3):187-91. PubMed ID: 2847912.
    Abstract:
    The rate of exchange of phosphoryl groups between 2-phosphoglycerate, 3-phosphoglycerate and phosphoenolpyruvate by the coupled phosphoglyceromutase-enolase enzyme system using one- and two-dimensional 31P NMR spectroscopy was measured. Magnetization exchange in one-dimensional experiments was achieved by saturation transfer with selective irradiation at both one and two sites in this three-site exchange system using the DANTE pulse sequence. The two-dimensional magnetization exchange experiment avoids the need to selectively saturate at one or more frequencies which may be difficult in complex exchange systems. Analysis of the two-dimensional exchange experiment by the back transformation method yielded exchange rate constants in good agreement with the saturation transfer method.
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