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  • Title: Reactivity of bilirubin photoisomers on the measurement of direct bilirubin using vanadic acid method.
    Author: Okada H, Itoh S, Kawamoto S, Ozaki M, Kusaka T.
    Journal: Ann Clin Biochem; 2018 Mar; 55(2):296-298. PubMed ID: 28485173.
    Abstract:
    Objective Investigation of the reactivity of fractions of bilirubin photoisomers with the vanadic acid oxidation method. Methods Bilirubin photoisomers were prepared by irradiating a bilirubin/human serum albumin solution with blue light emitting diode. Direct bilirubin and bilirubin fractions were measured using the vanadic acid oxidation method and high-performance liquid chromatography in the sample before and after irradiation. Results Direct bilirubin was increased in the solution containing bilirubin photoisomers. ( EE)-/( EZ) -cyclobilirubin-IXα and ( ZE)-/( EZ)-bilirubin-IXα completely disappeared after the addition of vanadic acid. Conclusion Bilirubin photoisomers reacted as direct bilirubin in the vanadic acid oxidation method.
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