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  • Title: A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium.
    Author: Michiels T, Popoff MY, Durviaux S, Coynault C, Cornelis G.
    Journal: Microb Pathog; 1987 Aug; 3(2):109-16. PubMed ID: 2849015.
    Abstract:
    A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
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