MEDLINE/PubMed Journal Browser Search

Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

Title: [The protection of hydrogen-rich saline on a rat dry eye model induced by scopolamine hydrobromide].
Author: Chu YY, Hua N, Ru YS, Zhao SZ.
Journal: Zhonghua Yan Ke Za Zhi; 2017 May 11; 53(5):363-372. PubMed ID: 28494565.
Abstract:
Objective: To evaluate the effect of hydrogen-rich saline (HRS) on dry eye rats induced by subcutaneous injection of scopolamine hydrobromide. Methods: Experiment research. Thirty female Wistar rats at about six weeks old were randomly divided into the normal group, dry eye group, HRS eyedrops group, normal saline eyedrops group (NS), HRS intraperitoneal injection group and NS intraperitoneal injection group, with 5 rats in each group. The dry eye was induced by subcutaneous injection of scopolamine hydrobromide in the latter five groups. The clinical signs of dry eye such as tear volume (SⅠt), tear break-up time (BUT) and corneal epithelial fluorescein staining scores were evaluated on day 7, 14, 21 and 28. On the 28th day, ten eyes in each group were enucleated and processed for paraffin sections for HE, PAS and immunohistochemistry stainings. Analysis of variance was used to test the data, and independent samples t-test was used for comparison between the two groups. Two-way repeated measure ANOVA was used to compare the difference among groups at different time points, one-way ANOVA was used to test the comparisons of the clinical signs at one time, and LSD was used to for comparison between two groups. Results: Before and after the experiment of the day 7, 14, 21, 28, the values of SIt in HRS eyedrops group and HRS intraperitoneal injection group were respectively:(3.625±1.157),(3.313±0.704),(3.250±0.535),(3.313±0.372), (3.375±0.582)mm and (3.500±1.019), (2.893±0.656), (3.321±0.668), (3.179±0.575), (3.214±0.871)mm. The values of BUT were respectively: (2.750±0.707), (2.688±0.594), (2.813±0.753), (3.000±0.756), (2.750±0.707)s and (3.000±0.679), (2.321±0.464), (2.750±0.753), (3.214±0.699), (2.679±0.608)s. The values of fluorescein staining score were respectively: (6.250±0.707), (8.875±0.641), (8.750±0.707), (9.250±0.463), (8.250±1.282) and (6.000±0.679), (9.143±1.027), (8.857±0.770), (9.143±0.949), (8.500±0.760). The difference of SIt, BUT and fluorescein staining score between the groups was statistically significant on the 14th day(F=5.194, 3.894, 16.487, P<0.05), the values of SⅠt, BUT and fluorescein staining score of HRS eyedrops group showed significantly better than NS eyedrops group(P<0.05), HRS intraperitoneal injection group showed significantly better than NS intraperitoneal injection group(P<0.05), and there was no significant difference between HRS eyedrops group and HRS intraperitoneal injection group(P>0.05), which remained stable until the day 28. On the 28(th) day, HE, PAS and immunohistochemistry stainings showed the corneal and conjunctival epithelia became smooth and regular, the cell layer number decreased, the tissue hyperplasia and edema were lightened, and the size of goblet cells tended to be normal, and the squamous metaplasia and inflammation were relieved. In HRS eyedrops group and HRS intraperitoneal injection group. Conclusions: HRS eyedrops group and HRS intraperitoneal injection group can relieve the signs of dry eye, improve the pathological damage of cornea and conjunctiva, and protect the ocular surface of a rat dry eye model, which is better than NS groups from the 14(th) day. (Chin J Ophthalmol, 2017, 53: 363-372). 目的： 探讨富氢盐水对东莨菪碱诱导的大鼠干眼模型眼表的保护作用。 方法： 实验研究。6周龄Wistar健康雌性大鼠30只（60只眼），随机数字表法分为正常对照组、干眼组、富氢盐水滴眼组、生理盐水滴眼组、富氢盐水腹腔注射组和生理盐水腹腔注射组，每组各5只（10只眼）。皮下注射东莨菪碱制备干眼模型，并于7、14、21、28 d对各组大鼠行泪液分泌试验（SⅠt）、泪膜破裂时间（BUT）、角膜上皮荧光素钠染色评分。于28 d制作眼球石蜡切片，行HE染色、过碘酸-雪夫（PAS）染色及免疫组织化学染色。采用两因素重复测量方差分析比较各组不同时间点整体上的差异，采用单因素方差分析比较各组间同一时间点的差异，进一步两两比较采用LSD检验。 结果： 实验前及实验后7、14、21、28d的SⅠt值在富氢盐水滴眼组和富氢盐水腹腔注射组分别为（3.625±1.157）、（3.313±0.704）、（3.250±0.535）、（3.313±0.372）、（3.375±0.582）mm和（3.500±1.019）、（2.893±0.656）、（3.321±0.668）、（3.179±0.575）、（3.214±0.871）mm；BUT值分别为（2.750±0.707）、（2.688±0.594）、（2.813±0.753）、（3.000±0.756）、（2.750±0.707）s和（3.000±0.679）、（2.321±0.464）、（2.750±0.753）、（3.214±0.699）、（2.679±0.608）s；角膜上皮荧光素钠染色评分分别为（6.250±0.707）、（8.875±0.641）、（8.750±0.707）、（9.250±0.463）、（8.250±1.282）分和（6.000±0.679）、（9.143±1.027）、（8.857±0.770）、（9.143±0.949）、（8.500±0.760）分。14 d时各组大鼠的SⅠt、BUT、荧光素钠染色评分差异有统计学意义（F=5.194，3.894，16.487；P<0.05）；富氢盐水滴眼组和生理盐水滴眼组相比，差异均有统计学意义（P<0.05）；富氢盐水腹腔注射组和生理盐水腹腔注射组相比，差异有统计学意义（P<0.05）；富氢盐水滴眼组和富氢盐水腹腔注射组相比，差异无统计学意义（P>0.05）；至28 d始终保持稳定。28 d时取眼球，行HE染色、PAS染色和免疫组化染色，结果示富氢盐水滴眼组和富氢盐水腹腔注射组大鼠角结膜上皮均变平整规则，细胞层次减少，水肿减轻，杯状细胞大小趋于正常，鳞状上皮化生和炎性反应程度减轻。 结论： 富氢盐水滴眼或腹腔注射均可缓解大鼠干眼体征，改善大鼠角结膜组织病理损伤；从用药14 d起富氢盐水对干眼大鼠眼表的保护作用明显优于生理盐水。（中华眼科杂志，2017，53：363-372）.

[Abstract] [Full Text] [Related] [New Search]