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  • Title: Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins.
    Author: Zylicz M, Ang D, Liberek K, Yamamoto T, Georgopoulos C.
    Journal: Biochim Biophys Acta; 1988 Dec 20; 951(2-3):344-50. PubMed ID: 2850011.
    Abstract:
    Using highly purified bacteriophage lambda and E. coli replication proteins, we were able to reconstitute an in vitro system capable of replication ori lambda-containing plasmid DNA. The addition of a new E. coli factor, the grpE gene product, to this replication system reduced the level of dnaK protein required for efficient DNA synthesis by at least 10-fold, and also allowed the isolation of a stable DNA replication intermediate. Based on all available information, we propose a molecular mechanism for the action of the dnaK and grpE proteins during the prepriming reaction leading to lambda DNA synthesis.
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