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  • Title: Quantification of leukotriene B4 in synovial fluid by gas chromatography/tandem mass spectrometry.
    Author: Dawson M, McGee CM, Brooks PM, Vine JH, Watson TR.
    Journal: Biomed Environ Mass Spectrom; 1988 Sep; 17(3):205-11. PubMed ID: 2850829.
    Abstract:
    A procedure for the quantification of leukotriene B4 (LTB4) in synovial fluid has been developed using gas chromatography/tandem mass spectrometry based on selected reaction monitoring of the elimination of t-butyldimethylsilanol from the ions of m/z 431 and 438 in the negative ion chemical ionization mass spectra of the di-t-butyldimethylsilyl/pentafluorobenzyl derivatives of leukotriene B4 and the internal standard (2H8)leukotriene B4. The detection limit (approximately 10 pg ml-1) is sufficiently low to permit determination of LTB4 concentration in the synovial fluid of patients with various arthropathies. Single-stage mass spectrometry was found not to be selective enough to permit quantification of LTB4 in synovial fluid.
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