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  • Title: Hachimijiogan and Tokishakuyakusan enhance deoxyribonucleic acid alpha-nucleotidyltransferase activity in relationship to DNA synthesis by ovarian follicles via a cyclic AMP system.
    Author: Usuki S.
    Journal: Am J Chin Med; 1988; 16(1-2):37-46. PubMed ID: 2851265.
    Abstract:
    The ovaries resected from twenty-nine day old female rats primed with 10 IU of PMS for 48 hours were incubated for 120 minutes with 2-20 micrograms/ml of Hachimijiogan (HZ) and Tokishakuyakusan (TS). The animals in another group were injected intravenously with 20 micrograms of HZ and TS 48 hours after PMS injection, and 20 minutes later decapitated. Deoxyribonucleic acid (DNA) alpha-nucleotidyltransferase activity and cyclic AMP accumulation in ovarian tissue were significantly increased by HZ and TS. These results suggest that HZ and TS enhance DNA alpha-nucleotidyltransferase activity via cyclic AMP by ovarian follicles.
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