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  • Title: ESR and spin-trapping study of free radicals in gamma-irradiated solid lysozyme.
    Author: Inanami O, Kuwabara M, Sato F.
    Journal: Free Radic Res Commun; 1988; 5(1):43-9. PubMed ID: 2853115.
    Abstract:
    Free radicals produced by gamma-irradiation of solid lysozyme were investigated by a technique combining ESR, spin-trapping and enzymatic digestion. MNP and DMPO were used as spin-trapping reagents. The solid lysozyme was first gamma-irradiated and then dissolved in an aqueous solution containing the spin-trapping reagent to stabilize free radicals. The spin adducts of lysozyme were digested to oligopeptides to get ESR spectra having a well-resolved hyperfine structure. The ESR spectra obtained showed that carbon-centered radicals, -CH-, at the side chains of amino acids, and thiyl radicals, -CH2-S., at disulfide bridges were produced in gamma-irradiated solid lysozyme.
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