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  • Title: A highly selective long-wavelength fluorescent probe for hydrazine and its application in living cell imaging.
    Author: Hao Y, Zhang Y, Ruan K, Meng F, Li T, Guan J, Du L, Qu P, Xu M.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2017 Sep 05; 184():355-360. PubMed ID: 28531842.
    Abstract:
    A highly selective long-wavelength turn-on fluorescent probe has been developed for the detection of N2H4. The probe was prepared by conjugation the tricyanofuran-based D-π-A system with a recognizing moiety of acetyl group. In the presence of N2H4, the probe can be effectively hydrazinolysized and produce a turn-on fluorescent emission at 610nm as well as a large red-shift in the absorption spectrum corresponding to a color change from yellow to blue. The sensing mechanism was confirmed by HPLC, MS, UV-vis, emission spectroscopic and theoretical calculation studies. The probe displayed high selectivity and sensitivity for N2H4 with a LOD (limit of detection) of 0.16μM. Moreover, the probe was successfully utilized for the detection of hydrazine in living cells.
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