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  • Title: Granulocyte-macrophage colony formation in vitro using human non-phagocytic bone marrow cells.
    Author: Ohhara N, Okamura S, Hayashi S, Otsuka T, Niho Y.
    Journal: Res Exp Med (Berl); 1988; 188(6):405-9. PubMed ID: 2853434.
    Abstract:
    A technique employing silica particles was used to remove cells producing endogenous colony-stimulating factor (CSF) to allow measurement of the level of colony-forming units in culture (CFU-C) in human bone marrow cells. In comparison with the glass-adherence technique, this new approach resulted in a more complete degree of removal of CSF-producing cells and formation of more colonies. This method seems to be useful for performing an accurate assay of exogenous CSF.
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