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  • Title: Purification of cardiac (Na+,K+)-activated adenosine triphosphatase from rat.
    Author: Watanabe T, Tawada Y, Shigekawa M.
    Journal: Anal Biochem; 1988 Nov 15; 175(1):284-8. PubMed ID: 2854374.
    Abstract:
    A procedure is described for preparation of highly active (Na+,K+)-ATPase from rat heart which has a specific activity of 200-600 mumol Pi/mg/h. The procedure is simple and can be applied to small amounts of heart muscle (approximately 1 g). The ATPase activity was more than 90% sensitive to ouabain (at concentrations up to 1 mM). The ouabain sensitivity is biphasic with about 20% of the ATPase activity being inhibited at approximately 3 X 10(-7) M ouabain.
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