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  • Title: Rapid and selective detection of E. coli O157:H7 combining phagomagnetic separation with enzymatic colorimetry.
    Author: Zhang Y, Yan C, Yang H, Yu J, Wei H.
    Journal: Food Chem; 2017 Nov 01; 234():332-338. PubMed ID: 28551244.
    Abstract:
    Mammal IgG antibodies are normally used in conventional immunoassays for E. coli O157:H7, which could lead to false positive results from the presence of protein A producing S. aureus. In this study, a natural specific bacteriophage was isolated and then conjugated with magnetic beads as a capture element in a sandwich format for the rapid and selective detection of E. coli O157:H7. To the best of our knowledge, it was the first time to utilize a natural bacteriophage to develop a phagomagnetic separation combined with colorimetric assay for E. coli O157:H7. The method has an overall time less than 2h with a detection limit of 4.9×104CFU/mL. No interference from S. aureus was observed. Furthermore, the proposed method was successfully applied to detect E. coli O157:H7 in spiked skim milk. The proposed detection system provided a potential method for E. coli O157:H7 and other pathogenic bacteria in food samples.
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