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  • Title: Demonstration of adrenergic and dopaminergic receptors in cultured sympathetic neurons--their coupling to cAMP but not to the transmitter release process.
    Author: Wakade AR, Wakade TD, Bhave SV, Malhotra RK.
    Journal: Neuroscience; 1988 Dec; 27(3):1021-8. PubMed ID: 2855258.
    Abstract:
    Experiments were carried out on cultured sympathetic neurons of the chick embryo; first, to demonstrate the presence of adrenergic and dopaminergic receptors, and then to see if these receptors are involved in regulation of transmitter release. We show that alpha 2-agonists, norepinephrine, epinephrine and clonidine, had no effect on neuronal cyclic 3',5'-adenosine monophosphate content. Forskolin enhanced neuronal cyclic 3',5'-adenosine monophosphate from a control value of about 20 pmoles/mg protein to 150 pmoles/mg protein. In the presence of alpha 2-agonists and forskolin the cyclic 3,5'-adenosine monophosphate content increased between 340 and 430 pmoles/mg protein. The alpha 1-agonist, phenylephrine, had no such effect. The facilitatory effect of alpha 2-agonist on forskolin-stimulated cyclic 3',5'-adenosine monophosphate production was blocked by the alpha 2-antagonist, yohimbine, but not the alpha 1-agonist, prazosin. Dopamine did not affect neuronal cyclic 3',5'-adenosine monophosphate content, but forskolin-stimulated increase in cyclic 3',5'-adenosine monophosphate was further facilitated by dopamine, and this effect was blocked by haloperidol. Activation of neuronal alpha 2-receptors by norepinephrine, epinephrine and clonidine did not interfere with electrically induced release of tritium from [3H]-norepinephrine-loaded sympathetic neurons. However, if sympathetic neurons were co-cultured with heart cells, clonidine, norepinephrine and epinephrine markedly inhibited the stimulation-induced release. Yohimbine or phentolamine partially reversed the inhibitory effects of alpha 2-agonists. alpha 2-Agonists and -antagonists also modified stimulation-induced release of tritium from [3H]norepinephrine-loaded hearts of the chick embryo.(ABSTRACT TRUNCATED AT 250 WORDS)
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