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Title: [Comparative analysis of hepatitis B virus large protein, hepatitis B virus-DNA and Pre-S1 antigen in evaluating serum hepatitis B virus replication]. Author: Xia F, Xu YH, Zheng MJ, Wang XL. Journal: Zhonghua Yu Fang Yi Xue Za Zhi; 2017 Jun 06; 51(6):501-505. PubMed ID: 28592093. Abstract: Objective: To investigate the comparative study of serum hepatitis B virus (HBV) large protein (HBV-LP) , HBV-DNA, and Pre S1 antigen (Pre S1-Ag) detection in the evaluation of serum HBV replication in patients with chronic hepatitis B. Methods: A total of 482 patients infected with chronic hepatitis B virus (CHB) were enrolled and the serums were collected in a hospital of Hefei city in Anhui province from June 2013 to March 2015. The serum HBV-LP, HBV markers(HBV-M) and Pre S1-Ag were detected using ELISA, and HBV-DNA were quantified using quantitative real-time PCR. The positive detection rate difference of HBV-DNA, HBV-LP and Pre S1-Ag were compared, the correlation between the logarithm of HBV-DNA copies number and the absorbance value of HBV-LP was analyzed using Spearman rank correlation. Results: The positive rates of HBV DNA, HBV-LP, and Pre S1-Ag were 67.22% (324/482), 73.86% (356/482), and 37.34% (180/482), respectively (P<0.01). The positive rates of the three markers were 54.57% (185/339), 64.90% (220/339), and 27.73% (94/339), respectively, in 339 HBeAg-negative CHB patients (P<0.01). In HBeAg negative patients, the positive rate of HBV-LP in 185 cases of HBV-DNA positive samples was 90.81% (168/185), which was higher than that of Pre S1-Ag with rate of 39.46% (73/185) (P<0.01).The positive rate of HBV-LP was 33.77% (52/154) in 154 cases of patients with negative HBV-DNA whose positive rate was higher than Pre S1-Ag with positive rate of 13.64% (21/154)(P<0.01). The positive rates of HBV-DNA, HBV-LP and Pre S1-Ag in HBsAg, HBeAg and HBcAb positive groups were 97.04% (131/135), 94.81% (128/135), and 60.00% (81/135), (P<0.01); The positive rates of three indexes of HBsAg, HBeAb, HBcAb positive group were 53.74% (122/227), 63.88% (145/227), and 27.31% (62/227); The positive rates of three indexes of HBsAg and HBcAb positive group were 55.79% (53/95), 67.37% (64/95), and 28.42% (27/95) (P<0.01). The absorbance value of HBV-LP was positively related with the logarithm of HBV-DNA copies number (Spearman rank correlation coefficient was 0.908, P<0.01). Conclusion: There was a good correlation between HBV-LP and HBV-DNA load value, and could be used as an effective complement for the detection of HBV-DNA and HBV-M. Compared with Pre S1-Ag. 目的: 对比分析乙型肝炎病毒外膜大蛋白(HBV-LP)、乙型肝炎病毒DNA(HBV-DNA)和前S1抗原(Pre S1-Ag)评价慢性乙型肝炎(CHB)患者血清中乙型肝炎病毒复制状况的有效性。 方法: 收集2013年6月至2015年3月在合肥某医院就诊的482例CHB患者的血清标本,采用ELISA法检测HBV-LP、乙型肝炎病毒标志物(HBV-M)和Pre S1-Ag;实时荧光定量PCR检测HBV-DNA。比较HBV-DNA、HBV-LP、Pre S1-Ag阳性率的差异,采用Spearman秩相关分析HBV-DNA拷贝数的对数值和HBV-LP的吸光度值相关性。 结果: 482例CHB患者HBV-DNA、HBV-LP和Pre S1-Ag阳性率分别为67.22%(324/482)、73.86%(356/482)和37.34%(180/482)(P<0.01);339例HBeAg阴性CHB患者的3项指标阳性率分别为54.57%(185/339)、64.90%(220/339)和27.73%(94/339)(P<0.01)。在HBeAg阴性患者中,185例为HBV-DNA阳性,HBV-LP阳性率为90.81%(168/185),高于Pre S1-Ag[39.46%(73/185)] (P<0.01);154例患者HBV-DNA阴性,其HBV-LP阳性率为33.77%(52/154),也高于Pre S1-Ag的13.64%(21/154)(P<0.01)。HBsAg、HBeAg、HBcAb阳性患者HBV-DNA、HBV-LP、Pre S1-Ag阳性率分别为97.04%(131/135)、94.81%(128/135)和60.00%(81/135)(P<0.01);HBsAg、HBeAb、HBcAb阳性患者3项指标阳性率分别为53.74%(122/227)、63.88%(145/227)和27.31%(62/227)(P<0.01);HBsAg、HBcAb阳性患者3项指标的阳性率分别为55.79%(53/95)、67.37%(64/95)和28.42%(27/95)(P<0.01)。HBV-LP的吸光度值随着HBV-DNA拷贝数的对数值增加而呈上升趋势(Spearman秩相关系数=0.908,P<0.01)。 结论: HBV-LP与HBV-DNA载量对数值之间的相关性良好,可作为HBV-DNA和HBV-M检测的有效补充,较Pre S1-Ag更能反映CHB患者的病毒复制状态。.[Abstract] [Full Text] [Related] [New Search]