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  • Title: A new simple phthalimide-based fluorescent probe for highly selective cysteine and bioimaging for living cells.
    Author: Shen Y, Zhang X, Zhang Y, Zhang C, Jin J, Li H.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2017 Oct 05; 185():371-375. PubMed ID: 28601704.
    Abstract:
    A new turn-on phthalimide fluorescent probe has designed and synthesized for sensing cysteine (Cys) based on excited state intramolecular proton transfer (ESIPT) process. It is consisted of a 3-hydroxyphthalimide derivative moiety as the fluorophore and an acrylic ester group as a recognition receptor. The acrylic ester acts as an ESIPT blocking agent. Upon addition of cystein, intermolecular nucleophilic attack of cysteine on acrylic ester releases the fluorescent 3-hydroxyphthalimide derivative, thereby enabling the ESIPT process and leading to enhancement of fluorescence. The probe displays high sensitivity, excellent selectivity and with large Stokes shift toward cysteine. The linear interval range of the fluorescence titration ranged from 0 to 1.0×10-5M and detection limit is low (6×10-8M). In addition, the probe could be used for bio-imaging in living cells.
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