These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Combination of cell penetrating peptides and heterologous DNA prime/protein boost strategy enhances immune responses against HIV-1 Nef antigen in BALB/c mouse model.
    Author: Kadkhodayan S, Jafarzade BS, Sadat SM, Motevalli F, Agi E, Bolhassani A.
    Journal: Immunol Lett; 2017 Aug; 188():38-45. PubMed ID: 28602843.
    Abstract:
    To develop a strong HIV specific T-cell response, the HIV-1 Tat and Nef regulatory proteins have been known as attractive antigenic candidates in vaccine design. A peptide transduction domain of Tat (48-60 aa) could act to deliver other therapeutic molecules into different cells. In this line, several cell-penetrating peptides (CPPs) have been designed to transfer DNA, siRNA, polypeptides and proteins into cells through non-covalent approach such as CADY and PEP families. Some studies showed that the endogenous adjuvants including heat shock protein Gp96 could stimulate antigen-specific T cell immune responses. In this study, different Nef DNA and protein constructs were generated, and their abilities were evaluated to induce T cell immune responses and humoral immunity in mouse model. A novel prime-boost immunization approach was also used in which the priming injections consisted of Nef/Tat (PTD)-Nef DNA plus Gp96 DNA followed by Nef/Tat (PTD)-Nef protein formulated in Freund's adjuvant or the Pep-1 and Cady-2 CPPs. Generally, our results indicated that Tat (PTD)-Nef fusion DNA or protein could significantly elicit higher humoral and cellular immune responses than Nef DNA or protein, respectively. Analysis of the immune responses demonstrated that the Tat (PTD)-Nef+Gp96 DNA prime/Tat (PTD)-Nef protein+Cady-2 boost regimen significantly enhanced the Nef/Tat (PTD)-Nef-specific T cell responses. This modality induced high levels of IgG2a and IFN-γ directed toward Th1 responses, and also Cytotoxic T Lymphocytes (CTLs) activity as compared to other immunization strategies. The immunostimulatory properties of Pep-1 and Freund's adjuvant were almost similar in different immunization strategies. These findings showed that the use of Tat (PTD)-Nef antigen in prime-boost strategy along with Gp96 adjuvant and Cady-2 CPP may have practical implications for developing HIV-1 vaccine in large animal model.
    [Abstract] [Full Text] [Related] [New Search]